Introduction flow cytometry learning guide
The purpose of this learning center is to connect scientists (whether new or experienced) to our many resources for learning about flow cytometry applications, techniques and basic principles by providing a few key points of entry into the vast content.Define key flow cytometry terms. Describe and explain key flow cytometry principles. Discuss the components and functions of the fluidics, optics, and electronics systems. Explain how data is analyzed. Describe the sorting process on flow cytometers. introduction flow cytometry learning guide
Introduction to Flow Cytometry: A Learning Guide A small percentage of this light is transmitted through the optic at the front (output coupler) of the tube. This transmitted light is the laser beam. The wavelength of the primary laser beam in benchtop ow cytometers is xed at 488 nm by placing a lter in front of the output coupler (Figure 71).
Flow cytometry computer software can generate data in the form of densityyp p plots and contour plots. These graphical representations can sometimes be misleading. Introduction to Flow Cytometry: A Learning Guide; Becton Dickinson Flow cytometry computer software can generate data in the form of densityyp p plots and contour plots. These graphical representations can sometimes be misleading. Introduction to Flow Cytometry: A Learning Guide; Becton Dickinsonintroduction flow cytometry learning guide Instead, we present a guide that will be invaluable to beginners in flow cytometry and act as a factpacked synopsis for those of you interested in teaching others about the virtues of this powerful application. To further assist learning we have recently added a Flow Cytometry glossary of terms to this guide.